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ABSTRACT

Back ground and Objective of the study: The present study aims to investigate in vivo antioxidant activity of Orthosiphon stamineus. Plant extracts were prepared using successive solvent extraction process using petroleum ether, chloroform and ethanol.

Methods: Type 2 diabetic rats were treated with extracts at the doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg for 21 days and tested for changes in thiobarbituric acid reactive substances (TBARS), conjugated dienes, catalase and peroxidase levels.

Results: Significant increase in TBARS and conjugated dienes and reduction in catalase and peroxidase were observed. All the extracts could significantly decrease TBARS and conjugated dienes; significantly increase catalse and peroxidase levels. Dose dependent and potent antioxidant effect was observed with ethanolic and petroleum ether extracts. The results of this study highlighted the antioxidant potential of O. stamineus and can be useful for protecting oxidative damage of pancreatic cells.

Conclusion: The study highlighted that the mechanisms can prevent damage of other organs and is beneficial for prevention of diabetic associated complications.

 Key words: Orthosiphon stamineus, Type 2diabetes, in vivo antioxidant activity, TBARS, Conjugated dienes, Catalase, Peroxidase.

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